February 26, 2020

Quantifying secretory burden with Ribo-Seq

Strategy

Culture, harvest and lyse

Nuclease footprinting and ribosome recovery

Footprint fragment purification

Dephosporylation, linker ligation, and pooling

rRNA depletion using Ribo-Zero

Reverse transcription and circularization

rRNA depletion using DSN

Library construction PCR

Sequencing